Hotspot

psbZ-trnG-UCC

LSC · 38840-39504 · 665 bp

Back to Gilbertiodendron

Pi 0.0278

MarkerSeek score 70.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 665
variable_sites: 26
pi: 0.0278
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	797-877 bp	1.0	85.0
psbZ-trnG-UCC_p2	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	800-880 bp	1.0	83.5
psbZ-trnG-UCC_p3	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	793-873 bp	1.0	83.4
psbZ-trnG-UCC_p4	CTTCTCCTGATGGTTGGTCG	GGATAGCGGGAATTGAACCC	55.0	55.0	58.0	58.4	818-898 bp	1.0	83.2
psbZ-trnG-UCC_p5	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	798-878 bp	1.0	83.1