Hotspot

atpF-atpH

LSC · 13636-14219 · 584 bp

Back to Gilbertiodendron

Pi 0.0268

MarkerSeek score 77.20

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 584
variable_sites: 23
pi: 0.0268
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCAGC	AGGCAGAGGGAAAAATACGG	50.0	50.0	58.1	57.6	768-780 bp	1.0	79.1
atpF-atpH_p2	CGTATGGCCAGTAACCCAAA	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	749-761 bp	1.0	79.1
atpF-atpH_p3	TTAAACCCGAAACTCCCAGC	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	58.1	57.5	772-784 bp	1.0	78.3
atpF-atpH_p4	CGTATGGCCAGTAACCCAAA	CCTGAGGCAGAGGGAAAAAT	50.0	50.0	57.9	57.5	753-765 bp	1.0	78.3
atpF-atpH_p5	TTAAACCCGAAACTCCCAGC	GCAGAGGGAAAAATACGGGG	50.0	55.0	58.1	58.6	766-778 bp	1.0	77.6