Hotspot

trnH-GUG-psbA

LSC · 86-457 · 372 bp

Back to Gilbertiodendron

Pi 0.0305

MarkerSeek score 67.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 16
pi: 0.0305
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCCAT	55.0	55.0	58.1	58.0	353-446 bp	1.0	86.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	367-460 bp	1.0	85.9
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	366-459 bp	1.0	85.9
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCCAT	50.0	55.0	57.9	58.0	368-461 bp	1.0	85.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	356-449 bp	1.0	85.5