Hotspot

petD

LSC · 77638-78882 · 1245 bp

Back to Ferula

Pi 0.00475
MarkerSeek score 53.10
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1245
variable_sites
33
pi
0.00475
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 ATTTCCCTTGAACACTGCAA TCCCTAGATACACAGGTCGT 40.0 50.0 55.7 56.9 1364-1372 bp 0.911765 52.2
petD_p2 TTTCCCTTGAACACTGCAAT TCCCTAGATACACAGGTCGT 40.0 50.0 55.7 56.9 1363-1371 bp 0.911765 52.2
petD_p3 ATTTCCCTTGAACACTGCAA GGGACAATTCGCTTTGTTTGA 40.0 42.9 55.7 57.6 1394-1402 bp 0.941176 51.5
petD_p4 TTTCCCTTGAACACTGCAAT GGGACAATTCGCTTTGTTTGA 40.0 42.9 55.7 57.6 1393-1401 bp 0.941176 51.5
petD_p5 TTTCCCTTGAACACTGCAAT AGGGACAATTCGCTTTGTTTG 40.0 42.9 55.7 57.6 1394-1402 bp 0.941176 51.5