Hotspot

rpl32-trnL-UAG

SSC · 120561-121475 · 915 bp

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Pi 0.00701
MarkerSeek score 62.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
915
variable_sites
59
pi
0.00701
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTAAGGGATATTGGGCAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1025-1081 bp 1.0 87.1
rpl32-trnL-UAG_p2 GTAAGGGATATTGGGCAGCC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1024-1080 bp 1.0 87.1
rpl32-trnL-UAG_p3 GGTAAGGGATATTGGGCAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1026-1082 bp 1.0 87.1
rpl32-trnL-UAG_p4 GTAAGGGATATTGGGCAGCC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1025-1081 bp 1.0 87.1
rpl32-trnL-UAG_p5 GTAAGGGATATTGGGCAGCC CTCGCACTGCTTCCTAAGAG 55.0 55.0 58.1 58.1 1045-1101 bp 1.0 86.5