Hotspot

ndhF-rpl32

SSC · 119266-120395 · 1130 bp

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Pi 0.00815
MarkerSeek score 60.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1130
variable_sites
88
pi
0.00815
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCGTGTGAACCAAAACCCTA AATTCCAGGCTTTTGTCGGT 50.0 45.0 58.0 58.0 2304-2433 bp 1.0 88.5
ndhF-rpl32_p2 CCGTGTGAACCAAAACCCTA ATTCCAGGCTTTTGTCGGTT 50.0 45.0 58.0 58.0 2303-2432 bp 1.0 88.5
ndhF-rpl32_p3 CCGTGTGAACCAAAACCCTA AGGCTTTTGTCGGTTGAGTT 50.0 45.0 58.0 57.9 2298-2427 bp 1.0 88.1
ndhF-rpl32_p4 AACCAAAATCCCCAACACGA AATTCCAGGCTTTTGTCGGT 45.0 45.0 57.9 58.0 2365-2494 bp 0.941176 84.0
ndhF-rpl32_p5 AACCAAAATCCCCAACACGA ATTCCAGGCTTTTGTCGGTT 45.0 45.0 57.9 58.0 2364-2493 bp 0.941176 84.0