Hotspot

rpl22-rps19

LSC · 84996-85073 · 78 bp

Back to Ferula

Pi 0.0432
MarkerSeek score 72.20
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
78
variable_sites
22
pi
0.0432
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.95
markerseek_score
72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 ACCACTCTTCTTGCTTTGTGA AAATTTCCGAGGACACGCAA 42.9 45.0 57.7 58.4 207-220 bp 0.970588 77.4
rpl22-rps19_p2 ACCACTCTTCTTGCTTTGTGA AATTTCCGAGGACACGCAAA 42.9 45.0 57.7 58.4 206-219 bp 0.970588 77.4
rpl22-rps19_p3 ACCACTCTTCTTGCTTTGTGA ATTTCCGAGGACACGCAAAA 42.9 45.0 57.7 58.4 205-218 bp 0.970588 77.4
rpl22-rps19_p4 ACCACTCTTCTTGCTTTGTGA TAAATTTCCGAGGACACGCA 42.9 45.0 57.7 57.5 208-221 bp 0.970588 76.9
rpl22-rps19_p5 ACCACTCTTCTTGCTTTGTGA TTTCCGAGGACACGCAAAAA 42.9 45.0 57.7 58.6 204-217 bp 0.970588 75.6