Hotspot

psbT-psbN

LSC · 75409-75469 · 61 bp

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Pi 0.0343
MarkerSeek score 70.60
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
61
variable_sites
9
pi
0.0343
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 ACGATCGAATCTATGGAAGCA AGAGATCCGTTCGAGGAACA 42.9 50.0 57.0 58.2 204-230 bp 1.0 69.0
psbT-psbN_p2 ACGATCGAATCTATGGAAGCA TCGAGGAACATGGGGACTAG 42.9 55.0 57.0 58.2 194-220 bp 1.0 68.9
psbT-psbN_p3 ACGATCGAATCTATGGAAGCA TTCGAGGAACATGGGGACTA 42.9 50.0 57.0 57.8 195-221 bp 1.0 68.7
psbT-psbN_p4 ACGATCGAATCTATGGAAGCA GTTTTACCGGGTACGCCTTA 42.9 50.0 57.0 57.6 257-283 bp 1.0 66.3
psbT-psbN_p5 ACGATCGAATCTATGGAAGCA GGGTACGCCTTATATACCGC 42.9 55.0 57.0 57.7 249-275 bp 0.970588 65.8