Hotspot

ycf3-trnS-GGA

LSC · 45434-46340 · 907 bp

Back to Ferula

Pi 0.00591
MarkerSeek score 68.80
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
907
variable_sites
55
pi
0.00591
unique_haplotype_count
30
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 766-1001 bp 1.0 79.5
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 787-1022 bp 1.0 78.9
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTCAA 47.6 45.0 58.0 57.8 737-972 bp 1.0 78.8
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA AGTTCCAATGCTACGCCTTC 47.6 50.0 58.0 58.3 739-974 bp 1.0 78.3
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 767-1002 bp 1.0 77.1