Hotspot

rpoB-trnC-GCA

LSC · 27399-28607 · 1209 bp

Back to Ferula

Pi 0.00507
MarkerSeek score 65.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1209
variable_sites
69
pi
0.00507
unique_haplotype_count
29
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCCTCATTTCCATCTCGGA CTTACCACTCGGCCATATCG 50.0 55.0 57.8 57.9 1235-1344 bp 1.0 85.5
rpoB-trnC-GCA_p2 TCCCTCATTTCCATCTCGGA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.8 58.1 1275-1384 bp 1.0 85.2
rpoB-trnC-GCA_p3 GTCAAGCCGTGATCCATGAA CTTACCACTCGGCCATATCG 50.0 55.0 58.3 57.9 1306-1415 bp 1.0 84.5
rpoB-trnC-GCA_p4 TCAAGCCGTGATCCATGAAC CTTACCACTCGGCCATATCG 50.0 55.0 58.3 57.9 1305-1414 bp 1.0 84.5
rpoB-trnC-GCA_p5 TCAAGCCGTGATCCATGAAC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.3 58.1 1345-1454 bp 1.0 84.2