Hotspot

rps16-trnQ-UUG

LSC · 5942-7385 · 1444 bp

Back to Ferula

Pi 0.0061
MarkerSeek score 65.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1444
variable_sites
87
pi
0.0061
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1515-1588 bp 1.0 86.5
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT AAATATAGATGGGGCGTGGC 45.0 50.0 58.0 57.8 1516-1589 bp 1.0 86.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AAATATAGATGGGGCGTGGC 45.0 50.0 58.1 57.8 1526-1599 bp 1.0 85.7
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1541-1614 bp 1.0 83.8
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG AAATATAGATGGGGCGTGGC 55.0 50.0 58.4 57.8 1540-1613 bp 1.0 83.8