Hotspot

trnK-UUU-rps16

LSC · 4094-4849 · 756 bp

Back to Ferula

Pi 0.00498
MarkerSeek score 65.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
756
variable_sites
42
pi
0.00498
unique_haplotype_count
26
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AGGAACTGTTCGGGATCTTT 50.0 45.0 58.0 56.5 864-906 bp 1.0 73.3
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 881-923 bp 1.0 72.9
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 880-922 bp 1.0 72.9
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 820-862 bp 1.0 72.7
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 819-861 bp 1.0 72.7