Hotspot

petD

LSC · 77560-78786 · 1227 bp

Back to Eremophila

Pi 0.0102

MarkerSeek score 62.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1227
variable_sites: 123
pi: 0.0102
unique_haplotype_count: 93
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGAGTTGAAGGGAATTCTCCG	CTTTGAAGCGCCTATTCCCT	52.4	50.0	58.4	58.0	80-1344 bp	0.994792	80.5
petD_p2	GGAGTTGAAGGGAATTCTCCG	TGAAGCGCCTATTCCCTAGA	52.4	50.0	58.4	57.9	80-1341 bp	0.994792	80.2
petD_p3	GAGTTGAAGGGAATTCTCCGA	CTTTGAAGCGCCTATTCCCT	47.6	50.0	57.4	58.0	80-1343 bp	0.994792	78.5
petD_p4	GAGTTGAAGGGAATTCTCCGA	TGAAGCGCCTATTCCCTAGA	47.6	50.0	57.4	57.9	80-1340 bp	0.994792	78.2
petD_p5	TGGAGTTGAAGGGAATTCTCC	CTTTGAAGCGCCTATTCCCT	47.6	50.0	57.3	58.0	80-1345 bp	0.994792	77.7