Hotspot

ndhF

SSC · 110900-113131 · 2232 bp

Back to Eremophila

Pi 0.0069
MarkerSeek score 51.10
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2232
variable_sites
291
pi
0.0069
unique_haplotype_count
132
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ATTTAGGCAGAACACCGTCC TTGCGCTTTGCCAATTCATT 50.0 40.0 57.9 57.8 80-2989 bp 0.796875 81.7
ndhF_p2 ATTTAGGCAGAACACCGTCC TTTGCGCTTTGCCAATTCAT 50.0 40.0 57.9 57.8 80-2990 bp 0.796875 81.7
ndhF_p3 ACACCGTCCCCCATTTTTAC ATTTGCGCTTTGCCAATTCA 50.0 40.0 58.1 57.8 80-2980 bp 0.770833 80.8
ndhF_p4 ACACCGTCCCCCATTTTTAC TTGCGCTTTGCCAATTCATT 50.0 40.0 58.1 57.8 80-2978 bp 0.770833 80.8
ndhF_p5 ACACCGTCCCCCATTTTTAC TTTGCGCTTTGCCAATTCAT 50.0 40.0 58.1 57.8 80-2979 bp 0.770833 80.8