Hotspot

rps2-rpoC2

LSC · 16852-17080 · 229 bp

Back to Eremophila

Pi 0.0212

MarkerSeek score 66.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 229
variable_sites: 46
pi: 0.0212
unique_haplotype_count: 161
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	TGCAGAGATATAGGGTGCCA	TCGATGATACATCAGAACAATCA	50.0	34.8	57.9	55.6	106-2120 bp	0.958333	50.3
rps2-rpoC2_p2	TGCAGAGATATAGGGTGCCA	TTCGATGATACATCAGAACAATCA	50.0	33.3	57.9	56.3	107-2121 bp	0.958333	50.3
rps2-rpoC2_p3	TGCAGAGATATAGGGTGCCA	CGATGATACATCAGAACAATCA	50.0	36.4	57.9	54.1	103-768 bp	0.958333	50.3
rps2-rpoC2_p4	CCTCCAGCATCTCTTCCAAG	TCGATGATACATCAGAACAATCA	55.0	34.8	57.7	55.6	80-2122 bp	0.947917	49.9
rps2-rpoC2_p5	AAATGAACTCCTGCCTCCAG	TCGATGATACATCAGAACAATCA	50.0	34.8	57.5	55.6	80-2119 bp	0.90625	48.3