Hotspot

trnK-UUU-rps16

LSC · 4290-5278 · 989 bp

Back to Eremophila

Pi 0.0112
MarkerSeek score 63.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
989
variable_sites
210
pi
0.0112
unique_haplotype_count
145
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 97-1997 bp 0.984375 76.7
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 97-1997 bp 0.984375 76.7
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 896-1996 bp 0.994792 76.3
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 100-1996 bp 0.984375 75.9
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 100-1996 bp 0.984375 75.9