Hotspot

ycf1

IRb · 112034-114302 · 2269 bp

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Pi 0.0017

MarkerSeek score 35.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 2269
variable_sites: 33
pi: 0.0017
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAACGAACGGGTTACCTAC	CAAGTCCCAACCCGAGTATG	55.0	55.0	58.0	58.0	2661-2779 bp	1.0	86.1
ycf1_p2	GGAACGAACGGGTTACCTAC	AAGTCCCAACCCGAGTATGA	55.0	50.0	58.0	58.1	2660-2778 bp	1.0	85.9
ycf1_p3	GGAACGAACGGGTTACCTAC	AGTCCCAACCCGAGTATGAA	55.0	50.0	58.0	58.1	2659-2777 bp	1.0	85.9
ycf1_p4	CGGGAGAATCAATGACTGCA	CAAGTCCCAACCCGAGTATG	50.0	55.0	58.0	58.0	2856-2974 bp	1.0	85.7
ycf1_p5	GGAACGAACGGGTTACCTAC	CAATGCCAACAGAAGCCAAG	55.0	50.0	58.0	57.9	2677-2795 bp	0.875	80.6