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infA-rps8

LSC · 82779-82859 · 81 bp

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Pi 0.0268

MarkerSeek score 66.20

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 81
variable_sites: 20
pi: 0.0268
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCTTCCTGAAAGATAACCCAGG	AATGACAGACCGAGAGACTC	50.0	50.0	58.1	56.4	197-268 bp	0.979167	61.5
infA-rps8_p2	CCTTCCTGAAAGATAACCCAGG	TTCTTTCCACTTCTCGAGGT	50.0	45.0	58.1	56.1	219-290 bp	1.0	60.0
infA-rps8_p3	AACCCAGGATCAGATCTTCA	AATGACAGACCGAGAGACTC	45.0	50.0	55.5	56.4	183-254 bp	0.979167	58.4
infA-rps8_p4	CCTTCCTGAAAGATAACCCAGG	AGGATTTTAGGAGGGATGGGA	50.0	47.6	58.1	57.5	312 bp	0.020833	12.3
infA-rps8_p5	AACCCAGGATCAGATCTTCA	AGGATTTTAGGAGGGATGGGA	45.0	47.6	55.5	57.5	298 bp	0.020833	9.3