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psbE-petL

LSC · 66650-67303 · 654 bp

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Pi 0.00339

MarkerSeek score 62.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 654
variable_sites: 31
pi: 0.00339
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GTCGTATCTTGCTCAGTCCA	50.0	50.0	57.9	57.1	844-910 bp	1.0	79.0
psbE-petL_p2	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGTCCA	45.0	50.0	58.2	57.1	853-919 bp	1.0	78.0
psbE-petL_p3	TGCTGACGAATAACCAACCC	GTCGTATCTTGCTCAGTCCA	50.0	50.0	58.2	57.1	858-924 bp	1.0	77.9
psbE-petL_p4	CGAATAACCAACCCGCAATG	GTCGTATCTTGCTCAGTCCA	50.0	50.0	57.8	57.1	852-918 bp	1.0	77.9
psbE-petL_p5	ACCAACCCGCAATGAATAGG	GTCGTATCTTGCTCAGTCCA	50.0	50.0	58.2	57.1	846-912 bp	1.0	77.5