Pi
0.00515
MarkerSeek score
59.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CTCTAACCGCTGAGCTAAGC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 866-921 bp | 1.0 | 86.3 |
| trnT-UGU-trnL-UAA_p2 | CTCTAACCGCTGAGCTAAGC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 874-929 bp | 1.0 | 85.8 |
| trnT-UGU-trnL-UAA_p3 | CAAATGCGATGCTCTAACCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.6 | 58.0 | 877-932 bp | 1.0 | 84.5 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 904-959 bp | 0.979167 | 84.5 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 912-967 bp | 0.979167 | 84.0 |