Hotspot

psaA-ycf3

LSC · 43058-43820 · 763 bp

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Pi 0.00775

MarkerSeek score 70.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 59
pi: 0.00775
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	904-935 bp	1.0	86.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	855-886 bp	1.0	85.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	918-949 bp	1.0	84.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	CAAGCCGCTGAGTATTGGAA	50.0	50.0	57.9	58.3	920-951 bp	1.0	84.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	TAGCGCTTACTCCGGGTAAT	50.0	50.0	57.9	58.3	892-923 bp	1.0	84.5