Hotspot

trnS-UGA-psbZ

LSC · 36693-37048 · 356 bp

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Pi 0.00167

MarkerSeek score 40.20

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 8
pi: 0.00167
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAAGACCGGAGCTATCAACC	CGCAAAAACAGCCAATTGGA	55.0	45.0	58.1	58.1	413-431 bp	1.0	84.0
trnS-UGA-psbZ_p2	CAAGACCGGAGCTATCAACC	GACCAACCCTCAGAAGAAGC	55.0	55.0	58.1	58.2	481-499 bp	1.0	83.5
trnS-UGA-psbZ_p3	CTATCAACCACTCGGCCATC	CGCAAAAACAGCCAATTGGA	55.0	45.0	58.1	58.1	402-420 bp	1.0	83.4
trnS-UGA-psbZ_p4	CTATCAACCACTCGGCCATC	GACCAACCCTCAGAAGAAGC	55.0	55.0	58.1	58.2	470-488 bp	1.0	83.0
trnS-UGA-psbZ_p5	CAAGACCGGAGCTATCAACC	CCAACCCTCAGAAGAAGCAA	55.0	50.0	58.1	57.7	479-497 bp	1.0	82.8