Hotspot

petN-psbM

LSC · 29066-30182 · 1117 bp

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Pi 0.00195

MarkerSeek score 60.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 30
pi: 0.00195
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCATTAA	TGTTCATTCTAATCCCTACGGC	45.0	45.5	58.1	57.9	1226-1261 bp	1.0	68.8
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGTTCATTCTAATCCCTACGGC	50.0	45.5	57.8	57.9	1232-1267 bp	1.0	67.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGTTCATTCTAATCCCTACGGC	50.0	45.5	57.6	57.9	1172-1207 bp	1.0	66.7
petN-psbM_p4	TCTTGCTTGGGCTGCATTAA	GTTCATTCTAATCCCTACGGCT	45.0	45.5	58.1	57.7	1225-1260 bp	1.0	66.6
petN-psbM_p5	TCTTGCTTGGGCTGCATTAA	ACGTAAAAACGGTCAGTCAA	45.0	40.0	58.1	55.6	1187-1222 bp	1.0	65.9