Hotspot

psbI-trnS-GCU

LSC · 8520-8684 · 165 bp

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Pi 0.0225

MarkerSeek score 77.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 165
variable_sites: 26
pi: 0.0225
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTATCGAATGATCCAGGGCG	ATTGGGAGAGATGGCTGAGT	55.0	50.0	58.0	58.1	209-314 bp	0.979167	89.6
psbI-trnS-GCU_p2	CGAATGATCCAGGGCGTAAT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.8	58.1	205-310 bp	1.0	89.5
psbI-trnS-GCU_p3	CTATCGAATGATCCAGGGCG	CTTCTTCGTACCGAGGGTTC	55.0	55.0	58.0	57.7	154-259 bp	0.979167	89.0
psbI-trnS-GCU_p4	CTATCGAATGATCCAGGGCG	GTGGACTAAAGCGGCTGATT	55.0	50.0	58.0	58.3	191-296 bp	0.979167	88.9
psbI-trnS-GCU_p5	CTATCGAATGATCCAGGGCG	TGGACTAAAGCGGCTGATTG	55.0	50.0	58.0	58.3	190-295 bp	0.979167	88.9