Hotspot

trnK-UUU-rps16

LSC · 4487-5957 · 1471 bp

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Pi 0.00551
MarkerSeek score 71.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1471
variable_sites
80
pi
0.00551
unique_haplotype_count
45
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT TGTTCTACGAACAAGGAAGCT 50.0 42.9 58.2 57.3 1369-1534 bp 0.9375 70.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT TGTTCTACGAACAAGGAAGCT 50.0 42.9 58.2 57.3 1368-1533 bp 0.9375 70.4
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC TGTTCTACGAACAAGGAAGCT 50.0 42.9 57.5 57.3 1355-1520 bp 0.9375 68.1
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TTGTTCTACGAACAAGGAAGCT 50.0 40.9 58.2 57.9 1370-1535 bp 0.9375 67.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TTGTTCTACGAACAAGGAAGCT 50.0 40.9 58.2 57.9 1369-1534 bp 0.9375 67.8