Hotspot

trnH-GUG-psbA

LSC · 152-583 · 432 bp

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Pi 0.0103

MarkerSeek score 57.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 432
variable_sites: 45
pi: 0.0103
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	465-501 bp	0.979167	89.2
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	466-502 bp	0.979167	89.0
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	530-566 bp	0.979167	88.5
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	GCGCTAACCTTGGTATGGAA	50.0	50.0	58.2	58.0	531-567 bp	0.979167	88.2
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	TATCATCAACCGCGCTAACC	50.0	50.0	57.9	58.1	541-577 bp	0.979167	87.5