Hotspot

ycf1

SSC · 124561-130203 · 5643 bp

Back to Clematis

Pi 0.00737

MarkerSeek score 69.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5643
variable_sites: 670
pi: 0.00737
unique_haplotype_count: 145
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTTGTTGATAACTTGGGCT	ACCGGATAATTCCAATGATGA	42.9	38.1	58.1	54.8	195-210 bp	0.925	49.0
ycf1_p2	TGCTTGTTGATAACTTGGGCT	CCGGATAATTCCAATGATGAAACA	42.9	37.5	58.1	57.6	194-209 bp	0.925	49.0
ycf1_p3	TGCTTGTTGATAACTTGGGCT	ACCGGATAATTCCAATGATGAAAC	42.9	37.5	58.1	57.6	195-210 bp	0.925	49.0
ycf1_p4	TGCTTGTTGATAACTTGGGCT	ACCGGATAATTCCAATGATGAA	42.9	36.4	58.1	55.6	195-210 bp	0.925	49.0
ycf1_p5	TGCTTGTTGATAACTTGGGC	ACCGGATAATTCCAATGATGA	45.0	38.1	56.6	54.8	195-210 bp	0.925	49.0