Hotspot

petB

LSC · 74557-75987 · 1431 bp

Back to Clematis

Pi 0.00523

MarkerSeek score 66.90

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1431
variable_sites: 119
pi: 0.00523
unique_haplotype_count: 115
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	CTATTCTGGCAGTTCGACCG	CAAACACATGTCTTATTCTTTTCA	55.0	29.2	58.4	54.3	1580-1621 bp	1.0	49.9
petB_p2	GCAGTTCGACCGTGAAATTC	CAAACACATGTCTTATTCTTTTCA	50.0	29.2	57.8	54.3	1572-1613 bp	0.96875	48.6
petB_p3	GCAGTTCGACCGTGAAATTC	CAAACACATGTCTTATTCTTTTCAA	50.0	28.0	57.8	55.0	1572-1613 bp	0.96875	48.6
petB_p4	GCAGTTCGACCGTGAAATTC	AAACACATGTCTTATTCTTTTCA	50.0	26.1	57.8	52.8	1571-1612 bp	0.96875	48.6
petB_p5	GCAGTTCGACCGTGAAATTC	AACACATGTCTTATTCTTTTCAA	50.0	26.1	57.8	52.8	1570-1611 bp	0.96875	48.6