Hotspot

ndhF

SSC · 110357-112591 · 2235 bp

Back to Clematis

Pi 0.00869

MarkerSeek score 56.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 265
pi: 0.00869
unique_haplotype_count: 133
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCCATCGTGGAACAATCAA	GCAACTCACTCAGTTTCCCA	50.0	50.0	58.2	58.0	2839-2957 bp	0.975	89.4
ndhF_p2	TTTGAATGGACCCCATCGTG	GCAACTCACTCAGTTTCCCA	50.0	50.0	58.2	58.0	2849-2967 bp	0.975	89.4
ndhF_p3	CCCCATCGTGGAACAATCAA	AACGGACTAGTTCACAACCG	50.0	50.0	58.2	57.9	2820-2938 bp	0.9625	88.0
ndhF_p4	TTTGAATGGACCCCATCGTG	AACGGACTAGTTCACAACCG	50.0	50.0	58.2	57.9	2830-2948 bp	0.9625	88.0
ndhF_p5	AAGGGGTCTCTTGCTATGGA	GCAACTCACTCAGTTTCCCA	50.0	50.0	57.7	58.0	2944-3000 bp	0.7	77.6