Hotspot

ndhE-ndhG

SSC · 118568-118844 · 277 bp

Back to Clematis

Pi 0.0289

MarkerSeek score 75.40

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 277
variable_sites: 81
pi: 0.0289
unique_haplotype_count: 118
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGACACATAAGCGCTCGAA	TGCTATGGCTCGTCAGTACT	45.0	50.0	57.6	58.2	276-434 bp	1.0	87.2
ndhE-ndhG_p2	AGACACATAAGCGCTCGAAC	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.4	58.2	275-433 bp	1.0	87.2
ndhE-ndhG_p3	CAAGACACATAAGCGCTCGA	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.4	58.2	277-435 bp	1.0	87.1
ndhE-ndhG_p4	TCAAGACACATAAGCGCTCG	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.4	58.2	278-436 bp	1.0	87.1
ndhE-ndhG_p5	GCTCAAGACACATAAGCGCT	TGCTATGGCTCGTCAGTACT	50.0	50.0	58.6	58.2	280-438 bp	1.0	85.0