Hotspot

psbE-petL

LSC · 64120-65499 · 1380 bp

Back to Clematis

Pi 0.00886

MarkerSeek score 72.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1380
variable_sites: 183
pi: 0.00886
unique_haplotype_count: 122
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	CCGCCAGTAGAAAACCGAAA	50.0	50.0	57.9	58.5	1450-1573 bp	1.0	87.7
psbE-petL_p2	ACGAATAACCAACCCGCAAT	CCGCCAGTAGAAAACCGAAA	45.0	50.0	58.2	58.5	1459-1582 bp	1.0	86.8
psbE-petL_p3	TGCTGACGAATAACCAACCC	CCGCCAGTAGAAAACCGAAA	50.0	50.0	58.2	58.5	1464-1587 bp	1.0	86.6
psbE-petL_p4	CGAATAACCAACCCGCAATG	CCGCCAGTAGAAAACCGAAA	50.0	50.0	57.8	58.5	1458-1581 bp	1.0	86.6
psbE-petL_p5	ACCAACCCGCAATGAATAGG	CCGCCAGTAGAAAACCGAAA	50.0	50.0	58.2	58.5	1452-1575 bp	1.0	86.2