Hotspot

rps16-trnK-UUU

LSC · 2342-2990 · 649 bp

Back to Clematis

Pi 0.0116
MarkerSeek score 72.70
Primer available yes
Label rps16-trnK-UUU

Feature Metadata

label_name
rps16-trnK-UUU
feature_type
igs
region
LSC
length_bp
649
variable_sites
132
pi
0.0116
unique_haplotype_count
103
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnK-UUU_p1 GCTCAACCTACGGAAACTGT TCTACCAACGGTATGGACGA 50.0 50.0 57.8 57.9 783-845 bp 1.0 89.7
rps16-trnK-UUU_p2 GCTCAACCTACGGAAACTGT AAAGCCGAGTACTCTACCGT 50.0 50.0 57.8 58.2 718-780 bp 1.0 89.3
rps16-trnK-UUU_p3 GCTCAACCTACGGAAACTGT AAGCCGAGTACTCTACCGTT 50.0 50.0 57.8 58.2 717-779 bp 1.0 89.3
rps16-trnK-UUU_p4 CCTTGAAAAAGGCGCTCAAC TCTACCAACGGTATGGACGA 50.0 50.0 58.2 57.9 796-858 bp 1.0 89.1
rps16-trnK-UUU_p5 CTTGAAAAAGGCGCTCAACC TCTACCAACGGTATGGACGA 50.0 50.0 58.2 57.9 795-857 bp 1.0 89.1