Hotspot

rpl32-trnL-UAG

SSC · 119147-120522 · 1376 bp

Back to Betula

Pi 0.00738
MarkerSeek score 53.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1376
variable_sites
48
pi
0.00738
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.96
markerseek_score
53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTACAGGGAATTCAAAAAGT AGCGTGTCTACCGATTTCAC 33.3 50.0 52.6 58.0 1051-1450 bp 1.0 46.8
rpl32-trnL-UAG_p2 TCTACAGGGAATTCAAAAAGT CAGCGTGTCTACCGATTTCA 33.3 50.0 52.6 58.0 1052-1451 bp 1.0 46.8
rpl32-trnL-UAG_p3 TCTACAGGGAATTCAAAAAGTT AGCGTGTCTACCGATTTCAC 31.8 50.0 53.5 58.0 1051-1450 bp 1.0 46.8
rpl32-trnL-UAG_p4 TTCTACAGGGAATTCAAAAAGT AGCGTGTCTACCGATTTCAC 31.8 50.0 53.5 58.0 1052-1451 bp 1.0 46.8
rpl32-trnL-UAG_p5 TCTACAGGGAATTCAAAAAGTT CAGCGTGTCTACCGATTTCA 31.8 50.0 53.5 58.0 1052-1451 bp 1.0 46.8