Hotspot

ndhF-rpl32

SSC · 118331-118990 · 660 bp

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Pi 0.0121
MarkerSeek score 64.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
660
variable_sites
61
pi
0.0121
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CACATGCGGCGAAGATTTTT ATGCTACCCAATACCCCTCC 45.0 55.0 58.0 58.6 854-1172 bp 1.0 84.9
ndhF-rpl32_p2 CACATGCGGCGAAGATTTTT GCTACCCAATACCCCTCCAT 45.0 55.0 58.0 58.6 852-1170 bp 1.0 84.9
ndhF-rpl32_p3 CACATGCGGCGAAGATTTTT TCAATGCTACCCAATACCCC 45.0 50.0 58.0 57.0 857-1175 bp 1.0 81.0
ndhF-rpl32_p4 CACATGCGGCGAAGATTTTT CAATGCTACCCAATACCCCT 45.0 50.0 58.0 56.9 856-1174 bp 1.0 80.9
ndhF-rpl32_p5 CACATGCGGCGAAGATTTTT GCTACCCAATACCCCTCCA 45.0 57.9 58.0 58.1 852-1170 bp 1.0 80.9