Hotspot

trnN-GUU-ndhF

IRb · 114241-116083 · 1843 bp

Back to Betula

Pi 0.0192
MarkerSeek score 56.60
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
IRb
length_bp
1843
variable_sites
97
pi
0.0192
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.90
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 TGAACCTACGACCAGTCAGT ATATAGGGGGTGGCCGAATT 50.0 50.0 58.0 58.2 1734-2094 bp 1.0 86.3
trnN-GUU-ndhF_p2 AAGACAGAGGGAACGGGTTA ATATAGGGGGTGGCCGAATT 50.0 50.0 58.0 58.2 1834-2194 bp 1.0 86.1
trnN-GUU-ndhF_p3 GGTATTGACGGTGATTCCCC ATATAGGGGGTGGCCGAATT 55.0 50.0 58.0 58.2 1904-2264 bp 1.0 85.8
trnN-GUU-ndhF_p4 GGCAGAATAGCAGAGCAAGT ATATAGGGGGTGGCCGAATT 50.0 50.0 58.0 58.2 2120-2480 bp 1.0 85.5
trnN-GUU-ndhF_p5 GTAATTCTTCCTCAGCCGGG ATATAGGGGGTGGCCGAATT 55.0 50.0 58.0 58.2 2144-2504 bp 1.0 85.3