Hotspot

petA-psbJ

LSC · 67955-69051 · 1097 bp

Back to Betula

Pi 0.00386
MarkerSeek score 55.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1097
variable_sites
25
pi
0.00386
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.0 57.6 1100-1250 bp 1.0 82.4
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1097-1247 bp 1.0 81.3
petA-psbJ_p3 TTACGTGTCCAAGGCCTTTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.6 57.6 1095-1245 bp 1.0 79.9
petA-psbJ_p4 ACGTGTCCAAGGCCTTTTAT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.4 57.6 1093-1243 bp 1.0 78.1
petA-psbJ_p5 ATTACGTGTCCAAGGCCTTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.4 57.6 1096-1246 bp 1.0 78.1