Hotspot

ndhC-trnV-UAC

LSC · 55187-56180 · 994 bp

Back to Betula

Pi 0.0354
MarkerSeek score 82.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
994
variable_sites
120
pi
0.0354
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.78
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCCGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 637-1125 bp 1.0 89.3
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 649-1137 bp 1.0 87.7
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCCGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 636-1124 bp 1.0 86.6
ndhC-trnV-UAC_p4 CTTCTCTGGCCCTTTGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 659-1147 bp 1.0 86.4
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCCGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 625-1113 bp 1.0 85.4