Hotspot

psaA-ycf3

LSC · 46177-46950 · 774 bp

Back to Betula

Pi 0.000664
MarkerSeek score 49.80
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
774
variable_sites
2
pi
0.000664
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
49.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TTGAGAAATGACCGGGTCTG ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.5 58.0 956-1007 bp 1.0 80.1
psaA-ycf3_p2 TTTTACTTCTGGTTCCGGCG ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.5 58.0 889-940 bp 1.0 80.0
psaA-ycf3_p3 TTGAGAAATGACCGGGTCTG CAGAGGCTTGGTTCGATCAA 50.0 50.0 57.5 57.9 971-1022 bp 1.0 79.7
psaA-ycf3_p4 TTTTACTTCTGGTTCCGGCG CAGAGGCTTGGTTCGATCAA 50.0 50.0 58.5 57.9 904-955 bp 1.0 79.5
psaA-ycf3_p5 TTGAGAAATGACCGGGTCTG CGATCAAGCCGCTGAGTATT 50.0 50.0 57.5 58.1 958-1009 bp 1.0 79.3