Hotspot

trnE-UUC-trnT-GGU

LSC · 34283-35280 · 998 bp

Back to Betula

Pi 0.00172
MarkerSeek score 55.80
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
998
variable_sites
13
pi
0.00172
unique_haplotype_count
16
primer_available
yes
alignment_reliability
0.91
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TTTCGTAGTACCCTACCCCC GAACCGATGACTTACGCCTT 55.0 50.0 57.8 58.0 1072-1181 bp 1.0 83.6
trnE-UUC-trnT-GGU_p2 TTTCGTAGTACCCTACCCCC CCATGGCGTTACTCTACCAC 55.0 55.0 57.8 58.1 1051-1160 bp 1.0 83.2
trnE-UUC-trnT-GGU_p3 TTTCGTAGTACCCTACCCCC TGGCGTTACTCTACCACTGA 55.0 50.0 57.8 58.1 1048-1157 bp 1.0 83.0
trnE-UUC-trnT-GGU_p4 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 1034-1143 bp 1.0 82.8
trnE-UUC-trnT-GGU_p5 TTTCGTAGTACCCTACCCCC ACTATGGTATGGCTGGGGTT 55.0 50.0 57.8 58.1 1125-1234 bp 0.952381 81.0