Hotspot

atpA-atpF

LSC · 13161-13215 · 55 bp

Back to Betula

Pi 0.0181
MarkerSeek score 51.90
Primer available yes
Label atpA-atpF

Feature Metadata

label_name
atpA-atpF
feature_type
igs
region
LSC
length_bp
55
variable_sites
3
pi
0.0181
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-atpF_p1 CCGTCGCCTACTTGAAGTAC TGTTTGGGACGCTGAAAGAA 55.0 45.0 58.1 58.0 200-205 bp 1.0 80.9
atpA-atpF_p2 CCGTCGCCTACTTGAAGTAC ATGTTTGGGACGCTGAAAGA 55.0 45.0 58.1 57.7 201-206 bp 1.0 79.0
atpA-atpF_p3 ACTTGAAGTACGGTACCGGT TGTTTGGGACGCTGAAAGAA 50.0 45.0 58.4 58.0 191-196 bp 1.0 78.7
atpA-atpF_p4 GTCGCCTACTTGAAGTACGG TGTTTGGGACGCTGAAAGAA 55.0 45.0 58.1 58.0 198-203 bp 0.952381 78.2
atpA-atpF_p5 GTCGCCTACTTGAAGTACGG ATGTTTGGGACGCTGAAAGA 55.0 45.0 58.1 57.7 199-204 bp 0.952381 76.4