Hotspot

trnS-GCU-trnG-UCC

LSC · 9113-10085 · 973 bp

Back to Betula

Pi 0.00787
MarkerSeek score 62.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
973
variable_sites
28
pi
0.00787
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AGGGATTAACAGAACGAATCACA 50.0 39.1 58.0 57.8 991-1069 bp 1.0 60.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AGGGATTAACAGAACGAATCACA 50.0 39.1 58.0 57.8 990-1068 bp 1.0 60.8
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GGGATTAACAGAACGAATCACAC 50.0 43.5 58.0 57.8 990-1068 bp 1.0 60.4
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGGATTAACAGAACGAATCACAC 50.0 43.5 58.0 57.8 989-1067 bp 1.0 60.4
trnS-GCU-trnG-UCC_p5 ACAAAAGCGGAAAGAGAGGG AGGGATTAACAGAACGAATCACA 50.0 39.1 58.1 57.8 1051-1129 bp 1.0 59.9