Hotspot

rps16-trnQ-UUG

LSC · 6831-7516 · 686 bp

Back to Betula

Pi 0.00369
MarkerSeek score 58.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
686
variable_sites
13
pi
0.00369
unique_haplotype_count
15
primer_available
yes
alignment_reliability
0.94
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CACAGCCGACCATATCCTTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.1 58.1 679-985 bp 1.0 83.7
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 659-965 bp 1.0 83.7
rps16-trnQ-UUG_p3 GTAAGAATCCACAGCCGACC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.3 58.1 688-994 bp 1.0 82.0
rps16-trnQ-UUG_p4 TGTAAGAATCCACAGCCGAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 689-995 bp 1.0 81.7
rps16-trnQ-UUG_p5 ACAGCCGACCATATCCTTCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.5 58.1 678-984 bp 1.0 80.7