Hotspot

ndhF-rpl32

SSC · 115263-116236 · 974 bp

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Pi 0.0145
MarkerSeek score 69.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
974
variable_sites
110
pi
0.0145
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAAAAGGTATGATCCACGCA AGCTTTTAAGGCTGCCCAAT 42.9 45.0 57.9 58.1 871-1145 bp 1.0 83.0
ndhF-rpl32_p2 ACAAAAGGTATGATCCACGCA AAGGCTGCCCAATATGCTTT 42.9 45.0 57.9 58.1 864-1138 bp 1.0 82.4
ndhF-rpl32_p3 ACAAAAGGTATGATCCACGCA AAAGCTTTTAAGGCTGCCCA 42.9 45.0 57.9 58.3 873-1147 bp 1.0 81.2
ndhF-rpl32_p4 ACAAAAGGTATGATCCACGCA AAGCTTTTAAGGCTGCCCAA 42.9 45.0 57.9 58.3 872-1146 bp 1.0 81.2
ndhF-rpl32_p5 ACAAAAGGTATGATCCACGCA GCTGCCCAATATGCTTTCCT 42.9 50.0 57.9 58.6 861-1135 bp 1.0 78.7