Hotspot

petA-psbJ

LSC · 64725-65661 · 937 bp

Back to Actinidia

Pi 0.0172

MarkerSeek score 77.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 937
variable_sites: 102
pi: 0.0172
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CCATTACGTGTTCAAGGCCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1028-1112 bp	1.0	87.2
petA-psbJ_p2	TCCATTACGTGTTCAAGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1029-1113 bp	1.0	87.2
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.8	57.6	995-1079 bp	1.0	87.1
petA-psbJ_p4	AGCATCTGTTGTTTTGGCAC	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	996-1080 bp	1.0	84.8
petA-psbJ_p5	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	1023-1107 bp	1.0	84.5