Hotspot

accD-psaI

LSC · 60274-60887 · 614 bp

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Pi 0.0134

MarkerSeek score 74.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 614
variable_sites: 69
pi: 0.0134
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTGAGTTATTTCAGTTCCACGA	CCATTGCAATTGCCGGAAAT	39.1	45.0	57.8	58.0	551-802 bp	1.0	65.8
accD-psaI_p2	AGTGAGTTATTTCAGTTCCACGA	ACGAAACCATTGCAATTGCC	39.1	45.0	57.8	58.2	557-808 bp	1.0	64.4
accD-psaI_p3	GAGTGAGTTATTTCAGTTCCACG	CCATTGCAATTGCCGGAAAT	43.5	45.0	57.5	58.0	552-803 bp	1.0	63.3
accD-psaI_p4	GTGAGTTATTTCAGTTCCACGA	CCATTGCAATTGCCGGAAAT	40.9	45.0	56.4	58.0	550-801 bp	1.0	62.7
accD-psaI_p5	AGTGAGTTATTTCAGTTCCACG	CCATTGCAATTGCCGGAAAT	40.9	45.0	56.4	58.0	551-802 bp	1.0	62.6