Hotspot

ndhC-trnV (UAC)

LSC · 51421-52604 · 1184 bp

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Pi 0.012

MarkerSeek score 71.30

Primer available yes

Label ndhC-trnV (UAC)

Feature Metadata

label_name: ndhC-trnV (UAC)
feature_type: igs
region: LSC
length_bp: 1184
variable_sites: 124
pi: 0.012
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV (UAC)_p1	GGGGCTAAAACTCCGGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1058-1350 bp	1.0	89.7
ndhC-trnV (UAC)_p2	CCTTTGCTAATCGGGGCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1070-1362 bp	1.0	88.0
ndhC-trnV (UAC)_p3	TCCTTTGCTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.5	58.1	1071-1363 bp	1.0	86.8
ndhC-trnV (UAC)_p4	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1063-1355 bp	1.0	85.9
ndhC-trnV (UAC)_p5	GGGGCTAAAACTCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1043-1335 bp	1.0	85.1