Hotspot

trnE (UUC)-trnT (GGU)

LSC · 31046-31841 · 796 bp

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Pi 0.00813

MarkerSeek score 67.30

Primer available yes

Label trnE (UUC)-trnT (GGU)

Feature Metadata

label_name: trnE (UUC)-trnT (GGU)
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 70
pi: 0.00813
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE (UUC)-trnT (GGU)_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	862-922 bp	1.0	88.4
trnE (UUC)-trnT (GGU)_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	859-919 bp	1.0	88.2
trnE (UUC)-trnT (GGU)_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	840-900 bp	1.0	87.7
trnE (UUC)-trnT (GGU)_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	837-897 bp	1.0	87.5
trnE (UUC)-trnT (GGU)_p5	CGTTGCCTCCTTGAAAGAGA	ACGACTTACGCCTTACCATG	50.0	50.0	57.8	57.7	877-937 bp	1.0	86.6