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trnC (GCA)-petN

LSC · 28312-29094 · 783 bp

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Pi 0.0125

MarkerSeek score 69.40

Primer available yes

Label trnC (GCA)-petN

Feature Metadata

label_name: trnC (GCA)-petN
feature_type: igs
region: LSC
length_bp: 783
variable_sites: 97
pi: 0.0125
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC (GCA)-petN_p1	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	817-867 bp	1.0	87.9
trnC (GCA)-petN_p2	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	878-928 bp	1.0	86.3
trnC (GCA)-petN_p3	TATCCATTTGGCGACATGGC	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.7	58.2	850-900 bp	1.0	85.3
trnC (GCA)-petN_p4	TATCCATTTGGCGACATGGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.7	57.6	911-961 bp	1.0	83.7
trnC (GCA)-petN_p5	AGTTCAAATCTGGGTGGCG	AGCCCAAGCGAGACTTACTA	52.6	50.0	58.1	58.2	792-842 bp	1.0	82.4