Hotspot

rpoB-trnC (GCA)

LSC · 26979-28230 · 1252 bp

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Pi 0.00759

MarkerSeek score 66.40

Primer available yes

Label rpoB-trnC (GCA)

Feature Metadata

label_name: rpoB-trnC (GCA)
feature_type: igs
region: LSC
length_bp: 1252
variable_sites: 97
pi: 0.00759
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC (GCA)_p1	CGTCAAGCCCTGATCAATGA	GTCTTTTGTTGATCAGGCGC	50.0	50.0	58.0	58.0	1308-1438 bp	1.0	89.2
rpoB-trnC (GCA)_p2	CGTCAAGCCCTGATCAATGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.0	58.4	1267-1397 bp	1.0	86.7
rpoB-trnC (GCA)_p3	AAAGTTCTTCCGTCAAGCCC	GTCTTTTGTTGATCAGGCGC	50.0	50.0	58.4	58.0	1318-1448 bp	1.0	86.2
rpoB-trnC (GCA)_p4	TTCTTTTTCATCCCGGAGCA	GTCTTTTGTTGATCAGGCGC	45.0	50.0	57.4	58.0	1233-1363 bp	1.0	85.0
rpoB-trnC (GCA)_p5	AAAGTTCTTCCGTCAAGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1277-1407 bp	1.0	83.7