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atpH-atpI

LSC · 13692-14775 · 1084 bp

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Pi 0.00771

MarkerSeek score 68.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1084
variable_sites: 79
pi: 0.00771
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	1164-1248 bp	1.0	82.6
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1181-1265 bp	1.0	81.6
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1174-1258 bp	0.977778	81.0
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.1	57.3	1149-1233 bp	1.0	80.9
atpH-atpI_p5	AGTACCCTGACCAACTCCAG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.4	58.5	1224-1308 bp	1.0	80.3